Cytoskeleton of Bovine Oocytes after Vitrification Using Ethylene Glycol Cryoprotectant
نویسندگان
چکیده
منابع مشابه
Current status of vitrification of embryos and oocytes in domestic animals: ethylene glycol as an emerging cryoprotectant of choice.
The cryopreservation of mammalian embryos has become an integral part of methods to control animal reproduction. Numerous vitrification solutions have been formulated with ethylene glycol in combination with macromolecules, sugars and other cryoprotective agents. These indicate that a study of ethylene glycol as a cryoprotectant of choice in vitrification studies would be promising. To understa...
متن کاملComparison of Ethylene Glycol and Propylene Glycol for the Vitrification of Immature Porcine Oocytes
Our aim was to optimize a cryoprotectant treatment for vitrification of immature porcine cumulus-oocyte complexes (COCs). Immature COCs were vitrified either in 35% ethylene glycol (EG), 35% propylene glycol (PG) or a combination of 17.5% EG and 17.5% PG. After warming, the COCs were in vitro matured (IVM), and surviving oocytes were in vitro fertilized (IVF) and cultured. The mean survival rat...
متن کاملthe vitrification of metaphase ii mouse oocyte using ethylene glycol as cryoprotectant
introduction: the purpose of this study was to investigate the effect of vitrification procedure on the survival, fertilization, development and ultrastructure of metaphase (mii) mouse oocyte. materials and methods: female nmri mice 6-10 weeks old were superovulated using ip injection of 10 iu hmg and 10 iu hcg. ovulated oocyte were collected from the ampullary portion of the oviducts at 12-13 ...
متن کاملHuman oocyte vitrification: the permeability of metaphase II oocytes to water and ethylene glycol and the appliance toward vitrification.
OBJECTIVE To determine the permeability of human metaphase II oocytes to ethylene glycol and water in the presence of ethylene glycol, and to use this information to develop a method to vitrify human oocytes. DESIGN An incomplete randomized block design. SETTING A university-affiliated assisted reproductive center. PATIENT(S) Women undergoing assisted reproduction in the Center for Reprod...
متن کاملCryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
Cryopreservation of mouse embryos is a technological basis that supports biomedical sciences, because many strains of mice have been produced by genetic modifications and the number is consistently increasing year by year. Its technical development started with slow freezing methods in the 1970s(1), then followed by vitrification methods developed in the late 1980s(2). Generally, the latter tec...
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ژورنال
عنوان ژورنال: Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences
سال: 2008
ISSN: 2502-5600,1978-225X
DOI: 10.21157/j.ked.hewan.v2i2.9780